Figure 1From: The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicityGranzyme B (GrB) secretion by αEN-EBV CTL cell line in the ELISPOT assay. Effector cells (104, 5 × 103 or 2.5 × 103 cells per well) were run against EN-EBV or K562 (NK control) targets, 5 × 104 cells per well, in a 4 h assay at 37°C. Data is presented as spots per well ± SD and is representative of 3 experiments. Similar results obtained for αJY CTL.Back to article page