Table 1 Quantitative real time PCR primers and probes for evaluating the four cytokines of interest and the reference genes.

β-actin

(f) 5'-GGCACCCAGCACAATGAAG-3'

(r) 5'-GCCGATCCACACGGAGTACT-3'

(p) 6FAM-TCAAGATCATTGCTCCTCCTGAGAGCGC-TAMRA

IFN-γ

(f) 5'-AGCTCTGCATCGTTTTGGGTT-3'

(r) 5'-GTTCCATTATCCGCTACATCTGAA-3'

(p) 6FAM-TCTTGGCTGTTACTGCCAGGACCCA-TAMRA

IL-2

(f) 5'-ACCAGGATGCTCACATTTAAGTTTT-3'

(r) 5'-GAGGTTTGAGTTCTTCTTCTAGACACTG-3'

(p) 6FAM-CATGCCCAAGAAGGCCACAGAACTG-TAMRA

IL-4

(f) 5'-GCGATATCACCTTACAGGAGATCAT-3'

(r) 5'-GTTACGGTCAACTCGGTGCA-3'

(p) 6FAM-TGCTGCCAACTGGCGCTCCTCC-TAMRA

IL-10

(f) 5'-GCCGTGGAGCAGGTGAAG-3'

(r) 5'-GAAGATGTCAAACTCACTCATGGCT-3'

(p) 6FAM-TGCCTTTAATAAGCTCCAAGAGAAAGGCATCTACA-TAMRA