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Table 1 Quantitative real time PCR primers and probes for evaluating the four cytokines of interest and the reference genes.

From: Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR)

Gene Primers and Probe
β-actin (f) 5'-GGCACCCAGCACAATGAAG-3'
  (r) 5'-GCCGATCCACACGGAGTACT-3'
  (p) 6FAM-TCAAGATCATTGCTCCTCCTGAGAGCGC-TAMRA
IFN-γ (f) 5'-AGCTCTGCATCGTTTTGGGTT-3'
  (r) 5'-GTTCCATTATCCGCTACATCTGAA-3'
  (p) 6FAM-TCTTGGCTGTTACTGCCAGGACCCA-TAMRA
IL-2 (f) 5'-ACCAGGATGCTCACATTTAAGTTTT-3'
  (r) 5'-GAGGTTTGAGTTCTTCTTCTAGACACTG-3'
  (p) 6FAM-CATGCCCAAGAAGGCCACAGAACTG-TAMRA
IL-4 (f) 5'-GCGATATCACCTTACAGGAGATCAT-3'
  (r) 5'-GTTACGGTCAACTCGGTGCA-3'
  (p) 6FAM-TGCTGCCAACTGGCGCTCCTCC-TAMRA
IL-10 (f) 5'-GCCGTGGAGCAGGTGAAG-3'
  (r) 5'-GAAGATGTCAAACTCACTCATGGCT-3'
  (p) 6FAM-TGCCTTTAATAAGCTCCAAGAGAAAGGCATCTACA-TAMRA
  1. (f) forward primer; (r) reverse primer; (p) probe * These primers and probes have been previously published [9, 19]