Figure 3

Effect of cell concentration on the specific kinetics of IFN-γ transcript production during CMVpp65 _495–503 peptide stimulation. Different concentrations of PBMCs from two CMV seropositive donors and one CMV seronegative donor were ex vivo peptide stimulated and analyzed at hour 3, 12 and 24 of sensitization for IFN-γ transcript production. For all donors tested, panels A, B and C show IFN-γ transcript production for each time of exposure (hour 3, 12 and 24, respectively) relative to cell concentration. Inversely, panels D, E and F show IFN-γ transcription for each quantity of stimulated cells relative to the three times of exposure only for the two seropositive donors. All results represent the mean ± SEM of three independent experiments carried out in triplicate.