IFN-γ transcript production by memory T lymphocytes following ex vivo FluM1
peptide stimulation. In the period of 3 to 12 hours, IFN-γ cytokine production was inversely proportional to the time of exposure during FluM158–66 stimulation (panel A), maintained an average level during CMVpp65495–503 stimulation (panel B) or was not produced during Mage12170–178 exposure (panel C). The results are expressed in mRNA copy numbers calculated relative to unstimulated cells after normalization by β-actin and represent the expressed values from each donor tested. Panel D specifically shows the kinetics of a representative donor (donor B) after the different viral peptide exposures relative to the negative value (unstimutated cells).