The kinetics of IFN-γ, IL-2, IL-4 and IL-10 transcription following FluM1
stimulation of three HLA-A*0201 donors. The standard concentration of 2 × 105cells/200μl was ex vivo exposed to the peptide. At hour 3, IFN-γ transcript production precedes IL-2, IL-4 and IL-10. At hour 12 there is consistent production of Th1 (IFN-γ, IL-2) cytokines. IL-4 and IL-10 transcript production increases above the baseline levels from 24 to 48 hours. For each donor and at each time point mRNA copy numbers were calculated relative to unstimulated cells after normalization by β-actin. The result represents the mean ± SEM of averages of combined values of three independent experiments carried out on each of the three donors.