Fig. 9

HIF-1α regulates LAMC1 expression in glioma cells. A, B Western blot and semi-quantitative analysis of LAMC1 and HIF-1α protein expression in glioma cells under normoxia (N) and hypoxia (H). C, D Western blot and semi-quantitative analyses of HIF-1α inhibitor YC-1-induced suppression of LAMC1 and HIF-1α protein expression in glioma cells at 12 h of hypoxia. ***P < 0.001. E Prediction of binding sites between the LAMC1 promoter and HIF-1α protein. F Results of luciferase reporter assays. ***P < 0.001; ns, no statistical difference. LAMC1^Pro-luc-HIF-1α-OE, LAMC1 promoter plus HIF-1α overexpression; LAMC1^Pro-luc-HIF-1α-NC, LAMC1 promoter plus negative control of HIF-1α; LAMC1^Pro-luc-NC-HIF-1α-OE, negative control of LAMC1 promoter plus HIF-1α overexpression; LAMC1^Pro-luc-NC-HIF-1α-NC, negative control of LAMC1 promoter plus negative control of HIF-1α