Fig. 1
From: Deciphering the importance of culture pH on CD22 CAR T-cells characteristics
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12967-024-05197-5/MediaObjects/12967_2024_5197_Fig1_HTML.png)
Characteristics of twenty CD22 CAR T-cell products manufactured at CCE of the NIH Clinical Center. a Viable total nucleated cells (TNC) count at the start of the CD22 manufacturing process (470 × 106) doubled over the time of the 9-day culturing process to 1.1 × 109 of viable TNC at the harvest. b Fold expansion rate reached its maximum on D7 of the manufacturing process (8.5) with a drop at the harvest (2.9). c CD3 percentage increased over the culturing process from 48% at apheresis bag to 99.7% of CD3+ at the harvest. d Transduction efficiency measured via flow cytometry reached 43% of protein L+ cells at the culture harvest. e CD4/CD8 ratio at the harvest