Fig. 5

AS-IV regulates the intestinal barrier function in colitic mice. (A) FITC-Dextran in the colon was detected as previously described. (B) FITC-Dextran in serum. (C) Transmission electron microscopy observation of TJs. (D) AB-PAS staining. (E) Quantitative analysis of (D). (F) Relative expression of Occludin, ZO-1, MUC2, Claudin-2, Claudin-5, and Claudin-7 in colon tissues. (G) Immunofluorescence detection of Villin, ZO-1, and Occludin expression in colon. (H) Quantitative analysis of (G). (I) Relative expression of Occludin, ZO-1, MUC2, Claudin-5, Claudin-7, and Claudin-2 in Caco-2 cells treated as indicated. Data indicate the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS or LPS group