Fig. 6

isiBCL-2 Exerts antitumor effects in vivo. A. Establishment of a xenograft nude mice model bearing human glioma U251 cells, with subsequent treatment using a specified amount of isiBCL-2. B. Removal, photography, and weighing of tumors after 22 days of RNA treatment. Tumor volume and mouse body weight were monitored every two days. C. Immunohistochemical (IHC) analysis, hematoxylin and eosin (H&E) staining, and TUNEL assay of tumors excised from U251 tumor-bearing mice. BCL-2 and Ki67 protein levels in the tumor tissue assessed using IHC staining. The apoptosis rate in each group was determined using H&E staining and TUNEL assay. Scale bar: 40 μm

Data are presented as mean ± standard deviation (n = 3). *p < 0.05 vs. PBS (control)