CDKN2B methylation is associated with carotid artery calcification in ischemic stroke patients
© The Author(s) 2016
Received: 15 August 2016
Accepted: 22 November 2016
Published: 1 December 2016
Cyclin-dependent kinase inhibitor 2A/2B (CDKN2A/2B) near chromosome 9p21 have been associated with both atherosclerosis and artery calcification, but the underlying mechanisms remained largely unknown. Considering that CDKN2A/2B is a frequently reported site for DNA methylation, this study aimed to evaluate whether carotid artery calcification (CarAC) is related to methylation levels of CDKN2A/2B in patients with ischemic stroke.
DNA methylation levels of CDKN2A/2B were measured in 322 ischemic stroke patients using peripheral blood leukocytes. Methylation levels of 36 CpG sites around promoter regions of CDKN2A/2B were examined with BiSulfite Amplicon Sequencing. CarAC was quantified with Agatston score based on results of computed tomography angiography. Generalized liner model was performed to explore the association between methylation levels and CarAC.
Of the 322 analyzed patients, 187 (58.1%) were classified as with and 135 (41.9%) without evident CarAC. The average methylation levels of CDKN2B were higher in patents with CarAC than those without (5.7 vs 5.4, p = 0.001). After adjustment for potential confounders, methylation levels of CDKN2B were positively correlated with cube root transformed calcification scores (β = 0.591 ± 0.172, p = 0.001) in generalized liner model. A positive correlation was also detected between average methylation levels of CDKN2B and cube root transformed calcium volumes (β = 0.533 ± 0.160, p = 0.001).
DNA methylation of CDKN2B may play a potential role in artery calcification.
KeywordsCarotid artery calcification CDKN2A/2B DNA methylation Ischemic stroke
As a surrogate measure of atherosclerosis, calcification may contribute to plaque vulnerability and, therefore, risk of vascular events . Because carotid bifurcation and adjacent segments are the predilection sites of atherosclerosis, calcification in these location can reflect the overall burden of vascular calcification , and may predict risk of stroke, myocardial infarction and the overall vascular events [3, 4].
Both functional  and genetic studies [13, 14] suggested that CDKN2A/2B may promote atherosclerosis by facilitating the process of calcification. But the mechanisms remain largely unknown. Considering that CDKN2A/2B is a frequently reported site of action for DNA methylation [15, 16], we hypothesized that DNA methylation in CDKN2A/2B may increase the susceptibility of artery calcification. In this study, we tested this hypothesis by evaluating the degree of DNA methylation in CDKN2A/2B and the carotid calcification load in a cohort of patients with ischemic stroke.
This study was approved by the Ethical Review Board of Jinling Hospital. Written informed consent was obtained from all enrolled patients. Consecutive patients with ischemic stroke were screened from Nanjing Stroke Registry Program  between July 2012 and September 2013. Patients were included if they: (1) were diagnosed with first-ever ischemic stroke within 7 days of onset; (2) aged 18 years or older; (3) completed a neck computed tomography angiography (CTA). Ischemic stroke was diagnosed if there were new focal neurological deficits explained by relevant lesions detected on diffusion-weighted imaging or computed tomography. Patients with malignant neoplasm, severe liver or kidney dysfunction, autoimmune diseases, parathyroid gland diseases, or calcium-phosphorus metabolic disorders were excluded. Since the stents may influence the accuracy of calcification assessment, patients with history of carotid artery stenting were also excluded. A total of 391 patients were screened and 324 patients were finally enrolled.
Artery calcification measurement
Each enrolled patient underwent a neck computed tomography angiography for CarAC evaluation. CTA was performed by a dual-source 64 slice CT system (Siemens, Forchheim, Germany) to quantify CarAC. Imaging was acquired by scanning from 4 cm below aortic arch to the superior border of orbit in craniocaudal direction. Details on CTA scan have been provided elsewhere .
Calcification scores in carotid artery were measured with Syngo Calcium Scoring system (Siemens, Forchheim, Germany). A focus of ≥4 contiguous pixels accompanied by a CT density ≥130 Hounsfield units (HU) was defined as calcification according to the method of Agatston score . Area of calcification (mm2) was multiplied by a weighted value assigned to its highest HU (130–199HU = 1; 200–299HU = 2; 300–399HU = 3; and >400HU = 4). Carotid calcification was measured at both sides within 3 cm proximal and distal segments of the bifurcation including four artery segments: common, bulb, internal, and external. The software used for calculating Agatston score also provided an isotropically interpolated calcium volume (mm3), by calculating the numbers of voxels with attenuation ≥130HU and summing the total voxel volumes. Calcification scores and calcium volume were assessed by two raters independently. The raters were blinded to other clinical data.
DNA isolation and epi-genotyping
Venous blood samples were drawn in the morning after an overnight fasting for biochemical marker assaying and methylation analyzing. Genomic DNA was extracted from whole blood with commercially available kits (TIANGEN Biotech, Beijing, China). DNA was quantified and then diluted to a working concentration of 10 ng/μL for genotyping.
Normality of parameters was assessed by Shapiro–Wilk test. As all continuous data in this study did not meet the normality assumption, they were described as median (interquartile range) and compared with Mann–Whitney U test. The non-parameters were compared with Fisher’s exact test. Patients were classified as without (Agatston score = 0), with mild (0 < Agatston score ≤ 100) and with severe (Agatston score > 100) CarAC. Methylation levels of CDKN2A/2B were compared between patients with and without CarAC using Mann–Whitney U test. Methylation levels of CDKN2A/2B were also compared among patients with mild, severe and without CarAC using Kruskal–Wallis test.
Spearman correlations were used to evaluate pairwise correlations of methylation levels between different CpG sites in the same gene. Given the heavily skewed distribution of calcification scores and calcium volume, cube root transformation was performed before comparison, as suggested in the previous studies [21, 22]. Generalized linear model was used to explore the association between methylation levels and cube root transformed calcification scores/calcium volumes after adjusting for age, sex, body mass index (BMI), diabetes mellitus (DM), hypertension (HTN) and smoking. These variables were chose for adjustment because they were identified as confounders that affected artery calcification. Bonferroni correction was used for multiple testing.
The data were analyzed by IBM SPSS Statistics Version 22.0 (Armonk, NY: IBM Corp.). A two-tailed value of p < 0.05 was considered statistically significant.
Comparison of demographic characteristics between patients with and without CarAC
All (n = 322)
With (n = 187)
Without (n = 135)
Male, n (%)
HTN, n (%)
DM, n (%)
CAD, n (%)
Smoking, n (%)
Drinking, n (%)
Based on Agatston score, 187 (58.1%) patients were grouped as with and 135 (41.9%) without CarAC. CarAC scores presented an extremely left-skewed distribution with a median (interquartile range) of 9.0 (0–111.1). The mean calcium volume (mm3) was 11.0 (0–98.0). Compared with patients without CarAC, those with CarAC were older (66.0 vs 57.0 years, p < 0.001), and had higher prevalences of HTN (82.9 vs 70.4%, p = 0.010) and DM (39.6 vs 26.7%, p = 0.017). Patients with CarAC had lower BMI (24.5 vs 24.9, p = 0.032), lower TC (4.17 vs 4.28 mmol/L, p = 0.029) and lower TG (1.36 vs 1.54 mmol/L, p = 0.016) levels (Table 1).
According to the results measured from target regions, there were 36 CpG sites (24 in CDKN2A and 12 in CDKN2B) identified as methylated sites (detailed information of each site was shown in Additional file 1: Table S2). The distribution of methylation levels of the 36 CpGs were listed in Additional file 1: Table S3. Methylation levels of CpG sites measured within CDKN2A were not significantly correlated, while those within CDKN2B were significantly correlated (Additional file 1: Table S4 and S5).
Differences of methylation levels (%) between patients with and without CarAC
Methylation levels of CDKN2A/2B according to severity of CarAC
Without (n = 135)
Mild (n = 103)
Sever (n = 84)
Association between methylation levels of CDKN2A/2B and cube root transformed calcification scores/calcium volumes
In this study, we observed a positive correlation between CDKN2B methylation and CarAC, which was quantified by Agatston score and calcium volume. These results verified our hypothesis that DNA methylation in CDKN2B may increase the susceptibility of artery calcification.
The relationship between Chr9p21 variants and artery calcification has been established previously [9, 23, 24]. Chr9p21 variants may up-regulate the expression of ANRIL, which was negatively correlated with the expression of CDKN2B . ANRIL can recruit and bind epigenetic modifiers such as polycomb repressor complex to promoter regions of adjacent genes [12, 15, 26]. These epigenetic regulations may eventually influence DNA methylation of CDKN2B. Methylation occurred in CpG islands around promoter regions generally inhibits gene expression . CDKN2B, known as a tumor suppressor, participates in cell cycle regulation via retinoblastoma (Rb) pathway . The protein p15INK4b, encoded by CDKN2B, can specifically bind to CDKN4 and CDKN6, resulting in G1 phase arrest and blockage of cell proliferation . The viewpoint that CDKN2B methylation may lead to unlimited cell proliferation has been verified in a spectrum of cancers [29, 30].
Chronic vascular inflammation arising from atherosclerosis contributes to calcification . Repression of CDKN2B may result in losing control of Rb proteins, which may subsequently enhance the proliferation of macrophage . In the condition of imbalance between promotion and inhibition of calcification, a proportion of VSMCs tend to differentiate into an osteoblastic and proliferative phenotype [31–33]. These processes play a role in the progression of arterial calcification. Therefore, methylation at CDKN2B may be a substantial contributor to artery calcification. And the possible association of CDKN2B methylation and atherosclerosis can be further extrapolated to patients with CAD or other cardiovascular diseases.
Our study has several strengths. To the best of our knowledge, this study was the first to report the association between CDKN2B methylation status and CarAC. CarAC was quantified by both Agatston method and calcium volume. Considering its less invasiveness and simplicity, methylation tests may be used in clinical settings for predicting the artery calcification. There are potential treatment implications. CpG island hypermethylation has been targeted in cancer treatment, with pharmacological agents modifying the epigenetic mechanisms been studied intensively . Similarly, agents which can specifically regulate CDKN2B methylation may be used for preventing artery calcification in future.
There are several limitations in our study. Firstly, the nature of the cross-sectional study limited us to reach a causal relationship. Secondly, the CDKN2A/2B expression was not evaluated in this study due to lack of fresh leukocytes. Further functional studies are warranted to clarify the underlying mechanisms that correlate CDKN2B methylation with artery calcification. Third, given the varied predisposition of DNA methylation in different tissues, methylation measured from leukocytes may not represent that of arterial wall. But considering that monocyte-derived macrophages, lymphocytes and platelets from peripheral blood are involved in atherogenesis , and harvesting vascular tissue from human body is largely impractical, the research strategy used in this study is logical and rational. Fourth, the present study was conducted in patients with ischemic stroke, which may generate selection bias. Not all potential confounders can be collected and analyzed due to the limited sample size and study resource. Moreover, patients with history of carotid artery stenting were excluded for accurate calcification assessment, which may lead to selection bias.
In summary, CDKN2B methylation is associated with CarAC independent of major cardiovascular risk factors. Our findings may enrich the body of knowledge on epigenetic pathology and provide some new implications for prevention and treatment of atherosclerotic diseases.
antisense noncoding RNA in the INK4 locus
body mass index
BiSulfite Amplicon Sequencing
carotid artery calcification
coronary artery disease
cyclin-dependent kinase inhibitor 2A/2B
computed tomography angiography
transcriptional start site
vascular smooth muscle cell
SZ and GX conceived and designed the experiments, and wrote the draft of the manuscript; LW and YZ assessed the calcification scores; YZ and SZ collected data; SZ and BC undertook the statistical analyses; SZ, BC, YZ, KL and HZ performed laboratory experiments; ZZ, LS, XX, MD and HC gave critical comments on the draft and contributed to the manuscript writing; GX, GL and XL reviewed clinical assessments in this study and supervised this study. All authors read and approved the final manuscript.
We thank all patients for participating in this study. We are also grateful to Center for Genetics & Genomic Analysis, Genesky Biotechnologies Inc. (Shanghai, 201203) for their technical support in sequencing.
The authors declare that they have no competing interests.
Availability of data and materials
The data will not be shared, since part of the data is being reused by another study.
Ethics approval and consent to participate
The study was approved by the Ethical Review Board of Jinling Hospital. Written informed consent was obtained from all enrolled patients.
This work was financially supported by the National Natural Science Foundation of China (NSFC, # 81571143 to GX, # 81530038 to XL and # 81220108008 to XL).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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