The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil
© Sánchez-Aragó and Cuezva; licensee BioMed Central Ltd. 2011
Received: 6 September 2010
Accepted: 8 February 2011
Published: 8 February 2011
Metabolic reprogramming resulting in enhanced glycolysis is a phenotypic trait of cancer cells, which is imposed by the tumor microenvironment and is linked to the down-regulation of the catalytic subunit of the mitochondrial H+-ATPase (β-F1-ATPase). The bioenergetic signature is a protein ratio (β-F1-ATPase/GAPDH), which provides an estimate of glucose metabolism in tumors and serves as a prognostic indicator for cancer patients. Targeting energetic metabolism could be a viable alternative to conventional anticancer chemotherapies. Herein, we document that the bioenergetic signature of isogenic colon cancer cells provides a gauge to predict the cell-death response to the metabolic inhibitors, 3-bromopyruvate (3BrP) and iodoacetate (IA), and the anti-metabolite, 5-fluorouracil (5-FU).
The bioenergetic signature of the cells was determined by western blotting. Aerobic glycolysis was determined from lactate production rates. The cell death was analyzed by fluorescence microscopy and flow cytometry. Cellular ATP concentrations were determined using bioluminiscence. Pearson's correlation coefficient was applied to assess the relationship between the bioenergetic signature and the cell death response. In vivo tumor regression activities of the compounds were assessed using a xenograft mouse model injected with the highly glycolytic HCT116 colocarcinoma cells.
We demonstrate that the bioenergetic signature of isogenic HCT116 cancer cells inversely correlates with the potential to execute necrosis in response to 3BrP or IA treatment. Conversely, the bioenergetic signature directly correlates with the potential to execute apoptosis in response to 5-FU treatment in the same cells. However, despite the large differences observed in the in vitro cell-death responses associated with 3BrP, IA and 5-FU, the in vivo tumor regression activities of these agents were comparable.
Overall, we suggest that the determination of the bioenergetic signature of colon carcinomas could provide a tool for predicting the therapeutic response to various chemotherapeutic strategies aimed at combating tumor progression.
Colorectal cancer (CRC) is a common neoplasia which poses a heavy burden on public health systems worldwide . Despite the establishment of CRC screening protocols, tailored therapeutic approaches are required to minimize the significant social impact of this disease . At present, KRAS mutation status is the only validated predictive marker for targeted CRC therapy . Thus, the development and clinical implementation of new predictive molecular markers are needed to aid in the selection of patients likely to respond to therapy and rationalized CRC treatments .
Cancer cells and tumors have a predominant glycolytic metabolism, even under aerobic conditions [3, 4]. Although the altered energetic metabolism of cancer cells has been proposed as a potential target for cancer treatment [3, 5–7], it could also represent a therapeutic obstacle, because of its contribution to chemo- and radio-resistance . In some tumors, this glycolytic phenotype is accompanied by a loss of bioenergetic activity in mitochondria [9, 10], which can be estimated by determining its bioenergetic signature[10, 11]. The bioenergetic signature is a protein ratio (β-F1-ATPase/GAPDH ratio) that assesses the expression of the catalytic subunit of mitochondrial H+-ATP synthase (β-F1-ATPase), a bottle-neck component required for the synthesis of biological energy, relative to the expression of glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) . Consistently, the bioenergetic signature has been observed to be significantly down-regulated in different human tumors compared to paired normal tissues [10, 12–19]. Recent findings indicate that the bioenergetic signature also represents a functional index of metabolic activity because it correlates, both in vivo and in vitro, with the rate of glucose utilization by cancer cells and tumors [9, 11]. Moreover, according to large cohort studies of colon [10, 19], lung [9, 14] and breast [16, 20] cancer patients, low tumor bioenergetic signatures are associated with poor patient prognosis, strongly suggesting that impaired mitochondrial bioenergetics is at the heart of cancer progression.
Remarkably, down-regulation of β-F1-ATPase has been widely associated with the resistance of cancer cells to standard anticancer therapies [21–23]. In the specific case of colon cancer cells, chemotherapeutic response to 5-fluorouracil (5-FU) [11, 21], as well as several metabolic inhibitors [23, 24], was assessed in cells with different genetic backgrounds: a condition that is likely to affect the cellular response to chemotherapeutic agents. The recent development of isogenic HCT116 colon cancer cell lines, representing different bioenergetic signatures, has provided an opportunity to unambiguously assess the influence of energetic metabolism on colon cancer therapy. In this study, we investigated cell death responses in metabolically different isogenic HCT116 cells and the regression of tumor xenographs, in response to the glycolytic inhibitors 3-bromopyruvate (3BrP) and iodoacetate (IA), and the classic chemotherapeutic agent, 5-FU. The small alkylating 3BrP and IA target the enzymes of glycolysis hexokinase  and GAPDH , respectively, although recent findings suggest that 3BrP also targets GAPDH .
Cell cultures and treatments
Human colorectal carcinoma HCT116 cells were grown in McCoy's 5A media supplemented with 10% fetal bovine serum. Twenty four h after seeding, cells were left untreated (M-type), treated with 6 μM oligomycin (G-type), or treated with 10 mM 2-DG (SM-type) for 48h. On the day of the experiment, culture medium was replaced without the addition of any drug and cells were used at ~ 60% confluence for experiments. Where indicated, cells were incubated with 10 μM 5-FU for 48h, or 8 μM 3BrP or 100 μM IA for 7h.
Protein electrophoresis and Western blot analysis
Cells were resuspended in lysis buffer (25 mM Hepes, 2.5 mM EDTA, 1% Triton X-100, 1 mM PMSF and 5 μg/mL leupeptin). Cell lysates were clarified by centrifugation at 11000 × g for 15 min. Resulting supernatants were fractionated on SDS-PAGE and transferred onto PVDF membranes for immunoblot analysis (Inmobilon-P, Millipore). Protein concentrations were determined using Bradford reagent (Bio-Rad protein assay). The primary monoclonal antibodies used were: anti-Hsp60 (Stressgene SPA-807, 1:2000) and anti-GAPDH (Abcam, 1:20000). The polyclonal rabbit anti-β-F1-ATPase (1:15000)  was also used. Peroxidase-conjugated anti-mouse or anti-rabbit IgGs (Nordic Immunonology, 1: 3000) were used as secondary antibodies. The blots were developed using the ECL reagent.
For determination of the rates of aerobic glycolysis, 0.1 mL aliquots of culture media were collected and used for enzymatic determination of lactate .
Cell death assays
Exposure of phosphatidylserine on the cell surface was analyzed after various cellular treatments using the annexin V-FITC assay (Sigma-Aldrich). Briefly, cells were washed twice in PBS and incubated in the dark for 10 min at room temperature with FITC-conjugated annexin-V (50 μg/mL) and propidium iodide (100 μg/mL) solutions. For each analysis, 10,000 events were recorded in a FACScan (Becton-Dickinson). Cell death was also determined using fluorescence microscopy. In brief, cells treated with the different compounds described were harvested, washed with PBS and incubated in the dark for 5 min at room temperature with Hoechst 33342 (1 mg/mL) and propidium iodide (1 mg/mL) solutions. After washing, samples were observed under a Leica DM-IRB fluorescence microscope (UV). The percentage of dead (red stained) cells was calculated from 10-20 different randomly selected fields for each condition assayed.
Caspase activity assays
Caspase 3/7 activity was determined using the luminogenic Ac-DEVD-pNA substrate included in the caspase-Glo 3/7 assay kit, according to the manufacturer's instructions (Promega). The reaction product was detected at 405 nm using a FLUOstar OPTIMA (BMG Labtech) plate luminometer.
Determination of ATP
Approximately 6 × 104 cells were seeded and treated as indicated. Cellular ATP concentrations were determined using an ATP Bioluminiscence Assay Kit (Roche).
In vivo tumorigenesis and treatments
Approximately, 1 × 107 G-type HCT116 cells were injected into the flank of 6-week-old male nude mice (National Cancer Institute, Frederick, Maryland). Tumor size was determined using a standard caliper and tumor volume was calculated using the formula: (width2 × length) × 0.52, where width represents the shortest dimension of the tumor . Twenty days after tumor induction, when tumors reached ~ 1,000 mm3 of volume, animals were randomly allocated into four different groups for daily intraperitoneal injections (100 μL) with inhibitors of glycolysis (8 μM 3BrP or 100 μM IA), a conventional treatment for colon cancer (0.5 mM 5-FU) or 0.9% NaCl as a control group. All treatments were performed for six consecutive days. Following treatment, animals were weighted and killed and the tumors extracted. All animal experiments were conducted according to the ethical rules established by the Universidad Autónoma de Madrid Review Board.
Statistical analysis was performed by Student's t test. Statistical tests were two-sided at the 5% level of significance. Pearson's correlation coefficient, p-value (p) and ANOVA with post hoc test (Dunnett's test) were calculated using the SPSS 17.0 software package.
In an effort to translate the bioenergetic signature to clinical practice, we have recently developed monoclonal antibodies against various markers of energetic metabolism . We found that cancer abolishes cell-type specific differences in the bioenergetic signature, supporting its use as a generic target to combat different neoplasias . Indeed, β-F1-ATPase expression has been shown to be a therapeutic response marker in different cancer cell lines, both for single and combined chemotherapy [19, 21–24, 33]. In the present study, we document the correlation between the bioenergetic signature of a cell, which represents an index of the relative relevance of cellular energy provision pathways [9, 11], and the potential to execute cell death in response to the metabolic inhibitors, 3BrP and IA, and the anti-metabolite 5-FU. The correlations observed in this study cannot be ascribed to differences in the genetic background of the cells because: (i) all of the cells were derived from the same parental HCT116 cells and (ii) the energetic metabolism of HCT116 cells is a reversible phenotypic trait amenable to regulation [11, 34]. Furthermore, although some cancer cells can oxidize glutamine for energy production purposes [3–5], glutamine contributes very little to the energetic metabolism of the highly glycolytic HCT116 cells used in this study. In fact, oxygen consumption rates, aerobic glycolysis rates and the bioenergetic signature of HCT116 cells are not affected by the presence of glutamine in the culture medium (see additional file 2).
Mechanistically, we propose that the cell death and tumor regression observed following administration of glycolytic inhibitors (3BrP and IA) may be due to induction of necrosis, whereas the cell death activity observed upon 5-FU treatment may occur through apoptosis (Figures 2 and 3). This later finding is consistent with the relevant roles played by oxidative phosphorylation  and mitochondrial H+-ATP synthase activity [33, 36] in the efficient execution of cell death. Indeed, the bioenergetic activity of mitochondria in colon cancer cells [11, 21] and tumors , has been associated with the ability to execute a ROS-mediated cell death response upon 5-FU treatment .
On the other hand, small alkylating agents have been shown to be able to kill cancer cells resistant to apoptosis by a process known as "programmed necrosis" through depletion of NAD+ via PARP1 activation . However, the induction of necrosis in response to the glycolytic inhibitors 3BrP and IA is exerted independently of PARP1 processing (data not shown), and most likely results from a metabolic catastrophe due to cellular ATP depletion (Figure 3) [23, 24]. Overall, our studies suggest that the enzymes of glycolysis could represent therapeutic targets for the treatment of colon cancer that may be as effective as conventional treatments (5-FU) at promoting tumor regression, in agreement with findings by others [25, 37].
The use of glycolytic inhibitors as chemotherapeutic agents has pros and contras. One problem is the deleterious effects that these agents could trigger in cell types strictly dependent on aerobic glycolysis for energy (e.g. neurons, lymphocytes, erythrocytes, retina, renal medulla, etc). However, glycolytic enzymes do have highly specific active site residues that, in principle, could provide more specific drug targets than those of proteins involved in signal transduction pathways. Thus, the use of such inhibitors may be beneficial in combination therapy as enhancers of the action of current chemotherapeutic drugs [7, 23]. Targeting energetic metabolism might represent an alternative cancer treatment route in the near future, because tumor cells that are resistant to chemotherapy could effectively die by necrosis in response to different metabolic inhibitors. Whatever the case, the bioenergetic signature offers a reliable gauge to predict the cell death response (apoptotic or necrotic cell death) to cancer therapy.
In summary, we have demonstrated that the bioenergetic signature of colon cancer cells inversely correlates with the potential to execute necrosis in response to treatments with glycolytic inhibitors. In contrast, the bioenergetic signature directly correlates with the apoptotic response to 5-FU treatment. Overall, our results support the use of the bioenergetic signature as a gauge for predicting cell death in response to different therapeutic strategies in colon cancer.
List of abbreviations
β catalytic subunit of the mitochondrial H+-ATP synthase
- bioenergetic signature :
Glyceraldehyde 3-phosphate dehydrogenase
We thank Mrs. M. Chamorro and C. Nuñez de Arenas for expert technical assistance and J. Palacín (CBMSO) for his support in nude mice studies. This work was supported by grants from the Ministerio de Educación y Ciencia (BFU2010-18903), the Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), ISCIII, Madrid and Comunidad de Madrid (S-GEN-0269), Spain. The CBMSO receives an institutional grant from the Fundación Ramón Areces.
- Gellad ZF, Provenzale D: Colorectal cancer: national and international perspective on the burden of disease and public health impact. Gastroenterology. 2010, 138: 2177-2190. 10.1053/j.gastro.2010.01.056.View ArticlePubMedGoogle Scholar
- Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal cancer. Lancet. 2010, 375: 1030-1047. 10.1016/S0140-6736(10)60353-4.View ArticlePubMedGoogle Scholar
- Cuezva JM, Ortega AD, Willers I, Sanchez-Cenizo L, Aldea M, Sanchez-Arago M: The tumor suppressor function of mitochondria: Translation into the clinics. Biochim Biophys Acta. 2009, 1792: 1145-1158.View ArticlePubMedGoogle Scholar
- DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB: The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab. 2008, 7: 11-20. 10.1016/j.cmet.2007.10.002.View ArticlePubMedGoogle Scholar
- Kroemer G, Pouyssegur J: Tumor cell metabolism: cancer's Achilles' heel. Cancer Cell. 2008, 13: 472-482. 10.1016/j.ccr.2008.05.005.View ArticlePubMedGoogle Scholar
- Tennant DA, Duran RV, Gottlieb E: Targeting metabolic transformation for cancer therapy. Nat Rev Cancer. 2010, 10: 267-277. 10.1038/nrc2817.View ArticlePubMedGoogle Scholar
- Pelicano H, Martin DS, Xu RH, Huang P: Glycolysis inhibition for anticancer treatment. Oncogene. 2006, 25: 4633-4646. 10.1038/sj.onc.1209597.View ArticlePubMedGoogle Scholar
- Diehn M, Cho RW, Lobo NA, Kalisky T, Dorie MJ, Kulp AN, Qian D, Lam JS, Ailles LE, Wong M: Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature. 2009, 458: 780-783. 10.1038/nature07733.PubMed CentralView ArticlePubMedGoogle Scholar
- Lopez-Rios F, Sanchez-Arago M, Garcia-Garcia E, Ortega AD, Berrendero JR, Pozo-Rodriguez F, Lopez-Encuentra A, Ballestin C, Cuezva JM: Loss of the mitochondrial bioenergetic capacity underlies the glucose avidity of carcinomas. Cancer Res. 2007, 67: 9013-9017. 10.1158/0008-5472.CAN-07-1678.View ArticlePubMedGoogle Scholar
- Cuezva JM, Krajewska M, de Heredia ML, Krajewski S, Santamaria G, Kim H, Zapata JM, Marusawa H, Chamorro M, Reed JC: The bioenergetic signature of cancer: a marker of tumor progression. Cancer Res. 2002, 62: 6674-6681.PubMedGoogle Scholar
- Sanchez-Arago M, Chamorro M, Cuezva JM: Selection of cancer cells with repressed mitochondria triggers colon cancer progression. Carcinogenesis. 2010, 31: 567-576. 10.1093/carcin/bgq012.View ArticlePubMedGoogle Scholar
- Bi X, Lin Q, Foo TW, Joshi S, You T, Shen HM, Ong CN, Cheah PY, Eu KW, Hew CL: Proteomic analysis of colorectal cancer reveals alterations in metabolic pathways: mechanism of tumorigenesis. Mol Cell Proteomics. 2006, 5: 1119-1130. 10.1074/mcp.M500432-MCP200.View ArticlePubMedGoogle Scholar
- Chen J, Kahne T, Rocken C, Gotze T, Yu J, Sung JJ, Chen M, Hu P, Malfertheiner P, Ebert MP: Proteome analysis of gastric cancer metastasis by two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-mass spectrometry for identification of metastasis-related proteins. J Proteome Res. 2004, 3: 1009-1016. 10.1021/pr049916l.View ArticlePubMedGoogle Scholar
- Cuezva JM, Chen G, Alonso AM, Isidoro A, Misek DE, Hanash SM, Beer DG: The bioenergetic signature of lung adenocarcinomas is a molecular marker of cancer diagnosis and prognosis. Carcinogenesis. 2004, 25: 1157-1163. 10.1093/carcin/bgh113.View ArticlePubMedGoogle Scholar
- He QY, Chen J, Kung HF, Yuen AP, Chiu JF: Identification of tumor-associated proteins in oral tongue squamous cell carcinoma by proteomics. Proteomics. 2004, 4: 271-278. 10.1002/pmic.200300550.View ArticlePubMedGoogle Scholar
- Isidoro A, Casado E, Redondo A, Acebo P, Espinosa E, Alonso AM, Cejas P, Hardisson D, Fresno Vara JA, Belda-Iniesta C: Breast carcinomas fulfill the Warburg hypothesis and provide metabolic markers of cancer prognosis. Carcinogenesis. 2005, 26: 2095-2104. 10.1093/carcin/bgi188.View ArticlePubMedGoogle Scholar
- Isidoro A, Martinez M, Fernandez PL, Ortega AD, Santamaria G, Chamorro M, Reed JC, Cuezva JM: Alteration of the bioenergetic phenotype of mitochondria is a hallmark of breast, gastric, lung and oesophageal cancer. Biochem J. 2004, 378: 17-20. 10.1042/BJ20031541.PubMed CentralView ArticlePubMedGoogle Scholar
- Unwin RD, Craven RA, Harnden P, Hanrahan S, Totty N, Knowles M, Eardley I, Selby PJ, Banks RE: Proteomic changes in renal cancer and co-ordinate demonstration of both the glycolytic and mitochondrial aspects of the Warburg effect. Proteomics. 2003, 3: 1620-1632. 10.1002/pmic.200300464.View ArticlePubMedGoogle Scholar
- Lin PC, Lin JK, Yang SH, Wang HS, Li AF, Chang SC: Expression of beta-F1-ATPase and mitochondrial transcription factor A and the change in mitochondrial DNA content in colorectal cancer: clinical data analysis and evidence from an in vitro study. Int J Colorectal Dis. 2008, 23: 1223-1232. 10.1007/s00384-008-0539-4.View ArticlePubMedGoogle Scholar
- Ortega AD, Sala S, Espinosa E, Gonzalez-Baron M, Cuezva JM: HuR and the bioenergetic signature of breast cancer: a low tumor expression of the RNA-binding protein predicts a higher risk of disease recurrence. Carcinogenesis. 2008, 29: 2053-2061. 10.1093/carcin/bgn185.View ArticlePubMedGoogle Scholar
- Shin YK, Yoo BC, Chang HJ, Jeon E, Hong SH, Jung MS, Lim SJ, Park JG: Down-regulation of mitochondrial F1F0-ATP synthase in human colon cancer cells with induced 5-fluorouracil resistance. Cancer Res. 2005, 65: 3162-3170.PubMedGoogle Scholar
- Li RJ, Zhang GS, Chen YH, Zhu JF, Lu QJ, Gong FJ, Kuang WY: Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance. Ann Oncol. 2010, 7: 1506-1514. 10.1093/annonc/mdp569.View ArticleGoogle Scholar
- Hernlund E, Hjerpe E, Avall-Lundqvist E, Shoshan M: Ovarian carcinoma cells with low levels of beta-F1-ATPase are sensitive to combined platinum and 2-deoxy-D-glucose treatment. Mol Cancer Ther. 2009, 8: 1916-1923. 10.1158/1535-7163.MCT-09-0179.View ArticlePubMedGoogle Scholar
- Hernlund E, Ihrlund LS, Khan O, Ates YO, Linder S, Panaretakis T, Shoshan MC: Potentiation of chemotherapeutic drugs by energy metabolism inhibitors 2-deoxyglucose and etomoxir. Int J Cancer. 2008, 123: 476-483. 10.1002/ijc.23525.View ArticlePubMedGoogle Scholar
- Geschwind JF, Ko YH, Torbenson MS, Magee C, Pedersen PL: Novel therapy for liver cancer: direct intraarterial injection of a potent inhibitor of ATP production. Cancer Res. 2002, 62: 3909-3913.PubMedGoogle Scholar
- Bickis IJ, Quastel JH: Effects of Metabolic Inhibitors on Energy Metabolism of Ehrlich Ascites Carcinoma Cells. Nature. 1965, 205: 44-46. 10.1038/205044a0.View ArticlePubMedGoogle Scholar
- Ganapathy-Kanniappan S, Geschwind JF, Kunjithapatham R, Buijs M, Vossen JA, Tchernyshyov I, Cole RN, Syed LH, Rao PP, Ota S, Vali M: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. Anticancer Res. 2009, 29: 4909-4918.PubMed CentralPubMedGoogle Scholar
- Pigault C, Follenius-Wund A, Schmutz M, Freyssinet JM, Brisson A: Formation of two-dimensional arrays of annexin V on phosphatidylserine-containing liposomes. J Mol Biol. 1994, 236: 199-208. 10.1006/jmbi.1994.1129.View ArticlePubMedGoogle Scholar
- Jaattela M: Multiple cell death pathways as regulators of tumour initiation and progression. Oncogene. 2004, 23: 2746-2756. 10.1038/sj.onc.1207513.View ArticlePubMedGoogle Scholar
- Zong WX, Ditsworth D, Bauer DE, Wang ZQ, Thompson CB: Alkylating DNA damage stimulates a regulated form of necrotic cell death. Genes Dev. 2004, 18: 1272-1282. 10.1101/gad.1199904.PubMed CentralView ArticlePubMedGoogle Scholar
- Munoz-Pinedo C, Ruiz-Ruiz C, Ruiz de Almodovar C, Palacios C, Lopez-Rivas A: Inhibition of glucose metabolism sensitizes tumor cells to death receptor-triggered apoptosis through enhancement of death-inducing signaling complex formation and apical procaspase-8 processing. J Biol Chem. 2003, 278: 12759-12768. 10.1074/jbc.M212392200.View ArticlePubMedGoogle Scholar
- Acebo P, Giner D, Calvo P, Blanco-Rivero A, Ortega AD, Fernandez PL, Roncador G, Fernandez-Malave E, Chamorro M, Cuezva JM: Cancer abolishes the tissue type-specific differences in the phenotype of energetic metabolism. Transl Oncol. 2009, 2: 138-145.PubMed CentralView ArticlePubMedGoogle Scholar
- Santamaria G, Martinez-Diez M, Fabregat I, Cuezva JM: Efficient execution of cell death in non-glycolytic cells requires the generation of ROS controlled by the activity of mitochondrial H+-ATP synthase. Carcinogenesis. 2006, 27: 925-935. 10.1093/carcin/bgi315.View ArticlePubMedGoogle Scholar
- Rossignol R, Gilkerson R, Aggeler R, Yamagata K, Remington SJ, Capaldi RA: Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells. Cancer Res. 2004, 64: 985-993. 10.1158/0008-5472.CAN-03-1101.View ArticlePubMedGoogle Scholar
- Tomiyama A, Serizawa S, Tachibana K, Sakurada K, Samejima H, Kuchino Y, Kitanaka C: Critical role for mitochondrial oxidative phosphorylation in the activation of tumor suppressors Bax and Bak. J Natl Cancer Inst. 2006, 98: 1462-1473. 10.1093/jnci/djj395.View ArticlePubMedGoogle Scholar
- Matsuyama S, Xu Q, Velours J, Reed JC: The Mitochondrial F0F1-ATPase proton pump is required for function of the proapoptotic protein Bax in yeast and mammalian cells. Mol Cell. 1998, 1: 327-336. 10.1016/S1097-2765(00)80033-7.View ArticlePubMedGoogle Scholar
- Fantin VR, St-Pierre J, Leder P: Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance. Cancer Cell. 2006, 9: 425-434. 10.1016/j.ccr.2006.04.023.View ArticlePubMedGoogle Scholar
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